ErbB2 / HER2 (phospho Y877) 抗体 [EP2324Y] (ab108371) (2024)

製品の概要

• 製品名

  Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y]
  ErbB2 / HER2 一次抗体 製品一覧

• 製品の詳細

  Rabbit monoclonal [EP2324Y] to ErbB2 / HER2 (phospho Y877)

• 由来種

  Rabbit

• アプリケーション

  適用あり: WB, IHC-P, Dot blot, ICC/IF, Flow Cyt (Intra)more details
  適用なし: IP

• 種交差性

  交差種: Human
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• ポジティブ･コントロール

  • HeLa cell lysate, SKBR-3 cell lysate, Human breast ductal infiltrating carcinoma tissue, SKBR-3 cells.

• 特記事項

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free production

  For more information see here.

  Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

• 製品の状態

  Liquid

• 保存方法

  Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• バッファー

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• Concentration information loading...

• 精製度

  Protein A purified

• ポリ/モノ

  モノクローナル

• クローン名

  EP2324Y

• アイソタイプ

  IgG

• 研究分野

  • Cell Type Markers
  • Tumor Associated

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Signal Transduction
  • Growth Factors/Hormones
  • EGF

  • Cancer
  • Growth factors
  • EGF

  • Cancer
  • Tumor immunology
  • Tumor-associated antigens

  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Neuroscience
  • Development

関連製品

• Alternative Versions

  • Alexa Fluor® 488 Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab237060)
  • Alexa Fluor® 647 Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] (ab237061)
  • Anti-ErbB2 / HER2 (phospho Y877) antibody [EP2324Y] - BSA and Azide free (ab238437)

• Isotype control

  • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

• Positive Controls

  • HeLa membrane extract lysate (ab29547)

アプリケーション

ターゲット情報

• 機能

  Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
  In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

• 組織特異性

  Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.

• 関連疾患

  Hereditary diffuse gastric cancer
  Glioma
  Ovarian cancer
  Lung cancer
  Gastric cancer
  Chromosomal aberrations involving ERBB2 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with CDK12, leading to CDK12-ERBB2 fusion leading to truncated CDK12 protein not in-frame with ERBB2.

• 配列類似性

  Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
  Contains 1 protein kinase domain.

• 翻訳後修飾

  Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).

• 細胞内局在

  Cytoplasm. Nucleus and Cell membrane. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1.

• Information by UniProt

• 参照データベース

  • Entrez Gene: 2064 Human
  • Omim: 164870 Human
  • SwissProt: P04626 Human
  • Unigene: 446352 Human

• 別名

  • Verb b2 erythroblastic leukemia viral oncogene hom*olog 2, neuro/glioblastoma derived oncogene hom*olog antibody
  • C erb B2/neu protein antibody
  • CD340 antibody

  see all

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データシートおよび資料

参考文献 (9)